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Article

Promoter-dependent disruption of genes: simple, rapid, and specific PCR-based method with application to three different yeast.

Current genetics - 1 Aug 2005

Edlind Thomas D, Henry Karl W, Vermitsky John-Paul, Edlind Merritt P, Raj Shriya, Katiyar Santosh K

Abstract excerpt

PCR product-based gene disruption has greatly accelerated molecular analysis of Saccharomyces cerevisiae. This approach involves amplification of a marker gene (e.g., URA3) including its flanking regulatory (promoter and polyadenylation) regions using primers that include at their 5' ends about 50 bases of homology to the targeted gene. Unfortunately, this approach has proved less useful in organisms with higher...

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PMID: 16078083
DOI: 10.1007/s00294-005-0008-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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