Molecular cloning of the gamma-glutamylcysteine synthetase gene of Saccharomyces cerevisiae.

doi:10.1002/yea.320070907

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Article

Molecular cloning of the gamma-glutamylcysteine synthetase gene of Saccharomyces cerevisiae.

Yeast (Chichester, England) - 1 Dec 1991

Ohtake Y, Yabuuchi S

Abstract excerpt

The 4.4 kb SphI DNA fragment (GSH1) that complements the gamma-glutamylcysteine synthetase-deficient mutation (gsh1) of Saccharomyces cerevisiae YH1 was cloned into vector plasmid YEp24. Gene disruption of the cloned fragment confirmed that this segment was the same gene as gsh1. Mutant strain YH1 with this plasmid not only restored gamma-glutamylcysteine synthetase (GSH-I) activity but the glutathione content...

Topics

Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Blotting, Southern
Chromosome Mapping
Cloning, Molecular
DNA, Fungal
Genetic Vectors
Glutamate-Cysteine Ligase
Kidney

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutation

Plasmids

Rats

Repetitive Sequences, Nucleic Acid

Restriction Mapping

Saccharomyces cerevisiae

Source

Open in PubMed

Identifiers

PMID: 1687097
DOI: 10.1002/yea.320070907

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1687097-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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