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Article

Cloning of a gene (PSD1) encoding phosphatidylserine decarboxylase from Saccharomyces cerevisiae by complementation of an Escherichia coli mutant.

The Journal of biological chemistry - 25 Nov 1993

Clancey C J, Chang S C, Dowhan W

Abstract excerpt

A gene (PSD1) encoding a phosphatidylserine decarboxylase of Saccharomyces cerevisiae was cloned by complementation of a conditional lethal mutation in the homologous gene in Escherichia coli strain EH150. Expression of the cDNA clone in EH150 corrected growth, phospholipid, and phosphatidylserine decarboxylase activity defects. Expression of the genomic clone in wild type yeast resulted in 20-fold amplification...

Topics

Amino Acid Sequence
Base Sequence
Carboxy-Lyases
Cloning, Molecular
DNA, Complementary
Escherichia coli
Genetic Complementation Test
Mitochondrial Proteins
Molecular Sequence Data
Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phenotype

Phospholipids

Ploidies

Saccharomyces cerevisiae

Sequence Homology, Amino Acid

Source

Identifiers

PMID: 8227017

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-8227017-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Cloning of a gene (PSD1) encoding phosphatidylserine decarboxylase from Saccharomyces cerevisiae by complementation of an Escherichia coli mutant.PMID 8227017Read ↗

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