Article
Cloning and bacterial expression of the CYS3 gene encoding cystathionine gamma-lyase of Saccharomyces cerevisiae and the physicochemical and enzymatic properties of the protein.
Journal of bacteriology - 1 Aug 1993
Yamagata S, D'Andrea R J, Fujisaki S, Isaji M, Nakamura K
Abstract excerpt
By screening a yeast genomic library, we isolated and characterized a gene rescuing the cysteine requirement in a "cys1" strain of Saccharomyces cerevisiae. Except for four residues in the open reading frame composed of 1,182 nucleotides, the DNA sequence was the same as that for the CYS3 (CYI1)...
Topics
- Acetyltransferases
- Base Sequence
- Cloning, Molecular
- Cystathionine gamma-Lyase
- Escherichia coli
- Gene Expression
- Genes, Fungal
- Kinetics
- Molecular Sequence Data
- Mutation
- Recombinant Proteins
- Saccharomyces cerevisiae
- Sequence Homology
- Serine O-Acetyltransferase