Molecular cloning of Drosophila gamma-glutamylcysteine synthetase by functional complementation of a yeast mutant.

doi:10.1016/s0014-5793(00)01148-0

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Article

Molecular cloning of Drosophila gamma-glutamylcysteine synthetase by functional complementation of a yeast mutant.

FEBS letters - 11 Feb 2000

Saunders R D, McLellan L I

Abstract excerpt

gamma-Glutamylcysteine synthetase (GCS) catalyses a critical, rate-limiting step in glutathione synthesis. In this study we describe the isolation and characterisation of a GCS cDNA (pDmGCS4.3. 3) from Drosophila melanogaster by functional complementation of a Saccharomyces cerevisiae gsh1 mutant. Expression of pDmGCS4.3.3 in the yeast mutant partially restored glutathione levels and conferred resistance to...

Topics

Amino Acid Sequence
Animals
Chromosome Mapping
Cloning, Molecular
DNA, Complementary
Drosophila melanogaster
Genetic Complementation Test
Glutamate-Cysteine Ligase
Glutathione
Molecular Sequence Data
Mutation
Phylogeny
Plasmids
Saccharomyces cerevisiae
Sequence Alignment

Source

Open in PubMed

Identifiers

PMID: 10675565
DOI: 10.1016/s0014-5793(00)01148-0

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