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Article

Characterization and structural analysis of human selenium-dependent glutathione peroxidase 4 mutant expressed in Escherichia coli.

Free radical biology & medicine - 1 Jun 2014

Yu Yang, Song Jian, Guo Xiao, Wang Shuan, Yang Xiao, Chen Long, Wei Jingyan

Abstract excerpt

Glutathione peroxidase 4 (GPx4) is a monomeric selenium-dependent glutathione peroxidase highly expressed in mammalian cells, which can reduce phospholipid hydroperoxides. However, it has been difficult to express recombinant mammalian GPx4 in Escherichia coli because of the differences in the selenocysteine (Sec) incorporation machinery between eukaryotes and prokaryotes. In this study, an E. coli BL21(DE3)cys...

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PMID: 24681209
DOI: 10.1016/j.freeradbiomed.2014.03.032

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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