Article
GSH2, a gene encoding gamma-glutamylcysteine synthetase in the methylotrophic yeast Hansenula polymorpha.
FEMS yeast research - 1 Aug 2002
Ubiyvovk Vira M, Nazarko Taras Y, Stasyk Olena G, Sohn Min Jeong, Kang Hyun Ah, Sibirny Andrei A
Abstract excerpt
The GSH2 gene, encoding Hansenula polymorpha gamma-glutamylcysteine synthetase, was cloned by functional complementation of a glutathione (GSH)-deficient gsh2 mutant of H. polymorpha. The gene was isolated as a 4.3-kb XbaI fragment that was capable of restoring GSH synthesis, heavy-metal resistance and cell proliferation when introduced into gsh2 mutant cells. It possesses 53% identical and 69% similar amino...
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