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Article

One-step site-directed mutagenesis of viroid dimeric cDNA.

Journal of virological methods - 1 Oct 2007

Sanjuán Rafael, Daròs José-Antonio

Abstract excerpt

The study of viroids (plant pathogens constituted by small non-coding RNA) has greatly benefited from the use of site-directed mutagenesis tools. However, compared to viral systems, this technique is complicated by the fact that, usually, infectious cDNAs carry two copies of the viroid genome. A simple method for a one-step site-directed mutagenesis of viroids is described and tested by estimating the rate of...

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Identifiers

PMID: 17604131
DOI: 10.1016/j.jviromet.2007.05.027

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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