Codon choice and potential complementarity between mRNA downstream of the initiation codon and bases 1471-1480 in 16S ribosomal RNA affects expression of glnS.

Abstract excerpt

A cis-acting expression mutation, GAG to GAA, in the third codon of the glnS gene is analyzed. Both codons code for glutamic acid but the mutation is known to increase gene expression by four fold. We show that the mutation has an effect only if it is located in the beginning of a gene but not if located internally. Data are presented that suggest that the reason for the increased expression by the mutation is...

Topics

• Arginine
• Base Composition
• Base Sequence
• Codon
• Escherichia coli
• Gene Expression Regulation, Bacterial
• Glutamate-tRNA Ligase
• Glutamates
• Glutamic Acid
• Molecular Sequence Data
• Mutation