Article
Frameshift suppression at tandem AGA and AGG codons by cloned tRNA genes: assigning a codon to argU tRNA and T4 tRNA(Arg).
Nucleic acids research - 11 Sept 1990
Spanjaard R A, Chen K, Walker J R, van Duin J
Abstract excerpt
Arginine is coded for by CGN (N = G, A, U, C), AGA and AGG. In Escherichia coli there is little tRNA for AGA and AGG and the use of these codons is strongly avoided in virtually all genes. Recently, we demonstrated that the presence of tandem AGA or AGG codons in mRNA causes frameshifts with high frequency. Here, we show that phaseshifts can be suppressed when cells are transformed with the gene for tRNA(T4Arg)...
Topics
- Base Sequence
- Cloning, Molecular
- Codon
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Protein Biosynthesis
- RNA, Messenger