Article
tRNA(2Gln) mutants that translate the CGA arginine codon as glutamine in Escherichia coli.
RNA (New York, N.Y.) - 1 Dec 1998
Tsai F, Curran J F
Abstract excerpt
We present a novel missense suppression system for the selection of tRNA(2GIn) mutants that can efficiently translate the CGA (arginine) codon as glutamine. tRNA(2Gln) mutants were cloned from a partially randomized synthetic gene pool using a plasmid vector that simultaneously expresses the tRNA...
Topics
- Alleles
- Amino Acyl-tRNA Synthetases
- Arginine
- Cloning, Molecular
- Codon
- Escherichia coli
- Genes, Suppressor
- Glutamine
- Mutagenesis, Site-Directed
- Nucleic Acid Conformation
- Polymerase Chain Reaction
- Protein Biosynthesis
- RNA, Transfer, Gln
- Structure-Activity Relationship
- beta-Galactosidase