Article
[Experimental study of epithelial-mesenchymal transition induced by HBx protein in liver cancer cell].
Zhonghua yi xue za zhi - 30 Mar 2010
Yang Shi-zhong, Zhang Ai-qun, Chen Geng, Zhang Lei-da, Zhu Jin, Li Xiao-wu, Dong Jia-hong
Abstract excerpt
OBJECTIVE: To explore whether or not HBx protein can induce epithelial-mesenchymal transition (EMT) in liver cancer cell SMMC-7721. METHODS: A recombinant adenovirus vector containing HBx gene was constructed and introduced into SMMC-7721 cell. The morphological changes were observed. The expression changes of epithelial and mesenchymal markers were investigated by RT-PCR, Western blot and immunohistochemistry....
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