Article
Unfolding time distribution of GFP by single molecule fluorescence spectroscopy.
European biophysics journal : EBJ - 1 Oct 2006
Chirico G, Cannone F, Diaspro A
Abstract excerpt
We have studied the unfolding of single molecules of GFP-mut2 mutant trapped in wet silica gels in a wide range of GuHCl concentration. After the addition of denaturant, the number of fluorescent molecules decreases with unfolding rates (of the order of 0.01 min(-1)) that are in very good agreeme...
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