Article
Single molecule spectroscopic characterization of GFP-MUT2 mutant for two-photon microscopy applications.
Microscopy research and technique - 1 Nov 2004
Cannone Fabio, Caccia Michele, Bologna Sara, Diaspro Alberto, Chirico Giuseppe
Abstract excerpt
Green Fluorescent Protein (GFP) mutants are extensively used in optical microscopy studies of in vivo biological processes in cells. Nonetheless, blinking and bleaching of the GFP chromophore at the single molecule level greatly limits its usefulness. We have worked out what we think are the best...
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