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Article

What causes hyperfluorescence: folding intermediates or conformationally flexible native states?

Biophysical journal - 1 Jul 2002

Ervin John, Larios Edgar, Osváth Szabolcs, Schulten Klaus, Gruebele Martin

Abstract excerpt

Hyperfluorescent intensity maxima during protein unfolding titrations are often taken as a sign for a thermodynamic folding intermediate. Here we explore another possibility: that hyperfluorescence could be the signature of a "pretransition" conformationally loosened native state. To model such native states, we study mutants of a fluorescent ubiquitin variant, placing cavities at various distances from the...

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Identifiers

PMID: 12080134
DOI: 10.1016/S0006-3495(02)75183-7
PMCID: PMC1302161

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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