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Article

Tracking unfolding and refolding of single GFPmut2 molecules.

Biophysical journal - 1 Sept 2005

Cannone Fabio, Bologna Sara, Campanini Barbara, Diaspro Alberto, Bettati Stefano, Mozzarelli Andrea, Chirico Giuseppe

Abstract excerpt

The unfolding and refolding kinetics of >600 single GFPmut2 molecules, entrapped in wet nanoporous silica gels, were followed by monitoring simultaneously the fluorescence emission of the anionic and neutral state of the chromophore, primed by two-photon excitation. The rate of unfolding, induced by guanidinium chloride, was determined by counting the number of single molecules that disappear in fluorescence...

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Identifiers

PMID: 15994904
DOI: 10.1529/biophysj.105.064584
PMCID: PMC1366706

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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