Article
Dynamics of green fluorescent protein mutant2 in solution, on spin-coated glasses, and encapsulated in wet silica gels.
Protein science : a publication of the Protein Society - 1 May 2002
Chirico Giuseppe, Cannone Fabio, Beretta Sabrina, Diaspro Alberto, Campanini Barbara, Bettati Stefano, Ruotolo Roberta, Mozzarelli Andrea
Abstract excerpt
Single-molecule experiments are performed by investigating spectroscopic properties of molecules either diffusing in and out of the observation volume or fixed in space by different immobilization procedures. To evaluate the effect of immobilization methods on the structural and dynamic properties of proteins, a highly fluorescent mutant of the green fluorescent protein, GFPmut2, was spectroscopically...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.