Article
Gene identification and cloning by molecular marker mapping.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2006
Jander Georg
Abstract excerpt
In the course of map-based cloning, mutant genes are identified through linkage to a sufficiently small region of the genetic map and subsequent DNA sequencing. This process has become fairly straightforward for Arabidopsis mutations, owing to the completed genome sequence and the discovery of many thousands of molecular markers. Initially, plants with the desired phenotype are identified in populations treated...
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