Article
Identification of components in disease-resistance signaling in Arabidopsis by map-based cloning.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2007
Zhang Yuelin, Glazebrook Jane, Li Xin
Abstract excerpt
With the whole genome sequence and thousands of defined polymorphisms between ecotypes available, it has become much easier to clone a gene by position (map-based cloning) in Arabidopsis. Recent development of DNA-isolation methods in plants also dramatically facilitated large-scale processing of DNA samples. Here, we describe detailed protocols for each step on general scheme of map-based cloning, from...
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