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Article

The Sequencing-Based Mapping Method for Effectively Cloning Plant Mutated Genes.

International journal of molecular sciences - 9 Jun 2021

Yu Li, Nie Yanshen, Jiao Jinxia, Jian Liufang, Zhao Jie

Abstract excerpt

A forward genetic approach is a powerful tool for identifying the genes underlying the phenotypes of interest. However, the conventional map-based cloning method is lengthy, requires a large mapping population and confirmation of many candidate genes in a broad genetic region to clone the causal variant. The whole-genome sequencing method clones the variants with a certain failure probability for multiple...

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Identifiers

PMID: 34207582
DOI: 10.3390/ijms22126224
PMCID: PMC8226582

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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