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Article

Generation and identification of Arabidopsis EMS mutants.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014

Qu Li-Jia, Qin Genji

Abstract excerpt

EMS mutant analysis is a routine experiment to identify new players in a specific biological process or signaling pathway using forward genetics. It begins with the generation of mutants by treating Arabidopsis seeds with EMS. A mutant with a phenotype of interest (mpi) is obtained by screening plants of the M2 generation under a specific condition. Once the phenotype of the mpi is confirmed in the next...

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PMID: 24057369
DOI: 10.1007/978-1-62703-580-4_12

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