Article
Mapping-by-Sequencing of Point and Insertional Mutations with Easymap.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022
Lup Samuel Daniel, Wilson-Sánchez David, Micol José Luis
Abstract excerpt
Random mutagenesis followed by screening for phenotypes of interest is a widely used strategy for genetic dissection of biological pathways; however, identifying the causal gene traditionally required time-consuming mapping approaches based on iterative linkage analysis. Mapping-by-sequencing accelerates this process, efficiently linking the phenotype of a mutant to a narrow candidate genomic region, using...
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