Article
Screening of mutations by TILLING in plants.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015
Wang Nian, Shi Lei
Abstract excerpt
TILLING (Targeting Induced Local Lesions IN Genomes) is a well-known reverse genetics technique designed to detect unknown SNPs (single nucleotide polymorphisms) in genes of interest using an enzymatic digestion and is widely employed in plant and animal genomics. The main advantage of this technique is that it allows for the high-throughput identification of an allelic series of mutants with a range of modified...
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