Article
Measuring intracellular redox conditions using GFP-based sensors.
Antioxidants & redox signaling - 1 Jan 2000
Björnberg Olof, Ostergaard Henrik, Winther Jakob R
Abstract excerpt
Recent years have seen the development of methods for analyzing the redox conditions in specific compartments in living cells. These methods are based on genetically encoded sensors comprising variants of Green Fluorescent Protein in which vicinal cysteine residues have been introduced at solvent-exposed positions. Several mutant forms have been identified in which formation of a disulfide bond between these...
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