Article
Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators.
The Journal of biological chemistry - 26 Mar 2004
Hanson George T, Aggeler Robert, Oglesbee Devin, Cannon Mark, Capaldi Roderick A, Tsien Roger Y, Remington S James
Abstract excerpt
Current methods for determining ambient redox potential in cells are labor-intensive and generally require destruction of tissue. This precludes single cell or real time studies of changes in redox poise that result from metabolic processes or environmental influences. By substitution of surface-exposed residues on the Aequorea victoria green fluorescent protein (GFP) with cysteines in appropriate positions to...
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