Article
Monitoring oxidative stress and DNA damage induced by heavy metals in yeast expressing a redox-sensitive green fluorescent protein.
Current microbiology - 1 May 2009
Yu Shanshan, Qin Wei, Zhuang Guoqiang, Zhang Xianen, Chen Guanjun, Liu Weifeng
Abstract excerpt
Reactive oxygen species (ROS) has been proposed to play an important role in heavy metal-associated toxicity and pathology. Conventional methods for determining ambient redox state in cells are usually labor-intensive, precluding real-time or single-cell monitoring changes in intracellular redox poise resulting from either metabolic processes or environmental influences. Redox-sensitive green fluorescent protein...
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