Protein kinase A-dependent inhibition of alkaline phosphatase release by SaOS-2 human osteoblastic cells: studies in new mutant cell lines that express a cyclic AMP-resistant phenotype.

doi:10.1091/mbc.2.11.889

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Article

Protein kinase A-dependent inhibition of alkaline phosphatase release by SaOS-2 human osteoblastic cells: studies in new mutant cell lines that express a cyclic AMP-resistant phenotype.

Cell regulation - 1 Nov 1991

Fukayama S, Kearns A K, Skurat R M, Tashjian A H, Bringhurst F R

Abstract excerpt

We have established mutant SaOS-2 cell lines that express a cyclic AMP (cAMP)-resistant phenotype to investigate the regulation and functional importance of orthophosphoric-monoester phosphohydrolase alkaline optimum (ALPase) in the action of parathyroid hormone (PTH). Cells were stably transfected with a plasmid that directs the synthesis of a mutant form of the type I regulatory subunit of protein kinase A...

Topics

Alkaline Phosphatase
Cell Line
Cyclic AMP
Enzyme Activation
Gene Expression Regulation
Humans
In Vitro Techniques
Ionomycin
Mutation
Osteoblasts
Parathyroid Hormone

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Kinase C

Protein Kinases

Receptors, Cyclic AMP

Signal Transduction

Transfection

Vasoactive Intestinal Peptide

Source

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Identifiers

PMID: 1667091
DOI: 10.1091/mbc.2.11.889
PMCID: PMC361887

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1667091-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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