Transfection-mediated expression of a dominant cAMP-resistant phenotype in the opossum kidney (OK) cell line prevents parathyroid hormone-induced inhibition of Na-phosphate cotransport. A protein kinase-A-mediated event.

doi:10.1172/JCI114860

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Article

Transfection-mediated expression of a dominant cAMP-resistant phenotype in the opossum kidney (OK) cell line prevents parathyroid hormone-induced inhibition of Na-phosphate cotransport. A protein kinase-A-mediated event.

The Journal of clinical investigation - 1 Nov 1990

Segal J H, Pollock A S

Abstract excerpt

Sodium-phosphate cotransport in the PTH-responsive opossum kidney (OK) cell line is inhibited by PTH, cAMP, and activators of protein kinase C. In order to probe the role of cAMP, we stably transfected OK cells with an expression vector for a cAMP-binding mutation of the murine protein kinase A regulatory subunit. Two-dimensional electrophoresis of cAMP-binding proteins from transfected cells indicated a 20-fold...

Topics

Animals
Biological Transport
Carrier Proteins
Cell Line
Cyclic AMP
Intracellular Signaling Peptides and Proteins
Kidney
Mice
Mutation
Opossums
Parathyroid Hormone

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Phenotype

Phosphates

Protein Kinases

Sodium

Sodium-Phosphate Cotransporter Proteins

Symporters

Transfection

Source

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Identifiers

PMID: 2173719
DOI: 10.1172/JCI114860
PMCID: PMC296888

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2173719-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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