Article
Skeletal alkaline phosphatase specific activity is an index of the osteoblastic phenotype in subpopulations of the human osteosarcoma cell line SaOS-2.
Metabolism: clinical and experimental - 1 Jul 1991
Farley J R, Hall S L, Herring S, Tarbaux N M, Matsuyama T, Wergedal J E
Abstract excerpt
During continuous culture with serial passage, the human osteosarcoma cell line SaOS-2 showed a time-dependent decrease in skeletal alkaline phosphatase (ALP) activity. Because this was indicative of heterogeneity, subpopulations of SaOS-2 cells were isolated from replicate low-density cultures. The subpopulations were less heterogeneous and more stable (with respect to ALP) than the parent population. ALP...
Topics
- Alkaline Phosphatase
- Bone and Bones
- Cyclic AMP
- Fluorides
- Histocytochemistry
- Humans
- Microbial Collagenase
- Osteoblasts
- Osteosarcoma
- Parathyroid Hormone
- Phenotype