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Article

Automated 3D light-sheet screening with high spatiotemporal resolution reveals mitotic phenotypes.

Journal of cell science - 1 Jun 2020

Eismann Björn, Krieger Teresa G, Beneke Jürgen, Bulkescher Ruben, Adam Lukas, Erfle Holger, Herrmann Carl, Eils Roland, Conrad Christian

Abstract excerpt

3D cell cultures enable the in vitro study of dynamic biological processes such as the cell cycle, but their use in high-throughput screens remains impractical with conventional fluorescent microscopy. Here, we present a screening workflow for the automated evaluation of mitotic phenotypes in 3D cell cultures by light-sheet microscopy. After sample preparation by a liquid handling robot, cell spheroids are imaged...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32295847
DOI: 10.1242/jcs.245043
PMCID: PMC7286290

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