Article
Physiological consequences of loss of allosteric activation of yeast NAD+-specific isocitrate dehydrogenase.
The Journal of biological chemistry - 23 Jun 2006
Hu Gang, Lin An-Ping, McAlister-Henn Lee
Abstract excerpt
Based on allosteric regulatory properties, NAD+-specific isocitrate dehydrogenase (IDH) is believed to control flux through the tricarboxylic acid cycle in vivo. To distinguish growth phenotypes associated with regulatory dysfunction of this enzyme in Saccharomyces cerevisiae, we analyzed strains expressing well defined mutant forms of IDH or a non-allosteric bacterial NAD+-specific isocitrate dehydrogenase...
Topics
- Allosteric Site
- Carbon
- Enzyme Activation
- Ethanol
- Gene Expression Regulation, Enzymologic
- Gene Expression Regulation, Fungal
- Genes, Fungal
- Genetic Complementation Test
- Isocitrate Dehydrogenase
- Kinetics
- Mutation
- Oxygen Consumption
- Saccharomyces cerevisiae
- Tricarboxylic Acids