Article
Isocitrate binding at two functionally distinct sites in yeast NAD+-specific isocitrate dehydrogenase.
The Journal of biological chemistry - 21 Jun 2002
Lin An-Ping, McAlister-Henn Lee
Abstract excerpt
Yeast NAD(+)-specific isocitrate dehydrogenase (IDH) is an octamer containing two types of homologous subunits. Ligand-binding analyses were conducted to examine effects of residue changes in putative catalytic and regulatory isocitrate-binding sites respectively contained in IDH2 and IDH1 subunits. Replacement of homologous serine residues in either subunit site, S98A in IDH2 or S92A in IDH1, was found to reduce...
Topics
- Adenosine Monophosphate
- Binding Sites
- Dose-Response Relationship, Drug
- Isocitrate Dehydrogenase
- Isocitrates
- Kinetics
- Ligands
- Models, Chemical
- Mutation
- NAD
- Plasmids
- Protein Binding
- Saccharomyces cerevisiae