Kinetic properties and metabolic contributions of yeast mitochondrial and cytosolic NADP+-specific isocitrate dehydrogenases.

doi:10.1074/jbc.M410140200

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Article

Kinetic properties and metabolic contributions of yeast mitochondrial and cytosolic NADP+-specific isocitrate dehydrogenases.

The Journal of biological chemistry - 11 Feb 2005

Contreras-Shannon Veronica, Lin An-Ping, McCammon Mark T, McAlister-Henn Lee

Abstract excerpt

To compare kinetic properties of homologous isozymes of NADP+-specific isocitrate dehydrogenase, histidine-tagged forms of yeast mitochondrial (IDP1) and cytosolic (IDP2) enzymes were expressed and purified. The isozymes were found to share similar apparent affinities for cofactors. However, with respect to isocitrate, IDP1 had an apparent Km value approximately 7-fold lower than that of IDP2, whereas, with...

Topics

Carbon
Carboxylic Acids
Catalysis
Cytosol
Electrophoresis
Genetic Complementation Test
Glucose
Glutamic Acid
Glycerol
Histidine
Hydrogen-Ion Concentration

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Immunoblotting

Isocitrate Dehydrogenase

Isocitrates

Ketoglutaric Acids

Kinetics

Lactates

NAD

Source

Open in PubMed

Identifiers

PMID: 15574419
DOI: 10.1074/jbc.M410140200

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