Article
Kinetic analysis of NAD(+)-isocitrate dehydrogenase with altered isocitrate binding sites: contribution of IDH1 and IDH2 subunits to regulation and catalysis.
Biochemistry - 14 Sept 1993
Cupp J R, McAlister-Henn L
Abstract excerpt
NAD(+)-dependent isocitrate dehydrogenase from Saccharomyces cerevisiae is an allosterically regulated enzyme that exists as an octamer composed of two nonidentical subunits, designated IDH1 and IDH2. To determine the contribution of each subunit to regulation and catalysis, a conserved serine re...
Topics
- Adenosine Monophosphate
- Allosteric Regulation
- Amino Acid Sequence
- Binding Sites
- Catalysis
- Enzyme Activation
- Isocitrate Dehydrogenase
- Isocitrates
- Kinetics
- Molecular Sequence Data
- Mutation
- Saccharomyces cerevisiae
- Sequence Homology, Amino Acid