[Deletion of two genes from the genome of fission yeast Schizosaccharomyces pombe. Genetic manipulation and phenotype study].

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Article

[Deletion of two genes from the genome of fission yeast Schizosaccharomyces pombe. Genetic manipulation and phenotype study].

Revista medico-chirurgicala a Societatii de Medici si Naturalisti din Iasi - 1 Jan 2000

Petrescu Elena, Voicu Pia-Maneula, Poiţelea M, Stoica B, Stănescu Raluca, Rusu M

Abstract excerpt

The aim of this study was to delete two genes from the genome of the fission yeast S. pombe in order to search for their functions in the cell. These genes are SPAC869.02c (MRI) and SPBC21C3.19 (MR2) and previous studies reported their significant induction after gamma irradiation. We carried out the deletions of the two genes and we replaced them with the selection marker ura4. Among the phenotype...

Topics

Gamma Rays
Gene Deletion
Gene Expression Regulation, Fungal
Genome, Fungal
Phenotype
Recombination, Genetic
Schizosaccharomyces
Ultraviolet Rays

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Open in PubMed

Identifiers

PMID: 16607836

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