A simple and effective chromosome modification method for large-scale deletion of genome sequences and identification of essential genes in fission yeast.

Abstract excerpt

The technologies for chromosome modification developed to date are not satisfactorily universal, owing to the typical requirements for special enzymes and sequences. In the present report, we propose a new approach for chromosome modification in Schizosaccharomyces pombe that does not involve any special enzymes or sequences. This method, designated the 'Latour system', has wide applicability with extremely high...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Chromosomes, Fungal
• Share to Gene Deletion