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Article

Gene disruption in Schizosaccharomyces pombe using a temperature-sensitive Ura4p.

Yeast (Chichester, England) - 15 Sept 1999

Davis K, Pateman C, Davey J

Abstract excerpt

We have generated a temperature-sensitive form of the Ura4p protein from the fission yeast Schizosaccharomyces pombe. A single T-to-C mutation at nucleotide 782 (relative to the initiator ATG codon of ura4) changes the leucine residue at position 261 in Ura4p to a proline. The mutant Ura4p(ts) supports growth at 30 degrees C but is unable to allow growth at 37 degrees C in the absence of uracil when a single copy...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 10487925
DOI: 10.1002/(SICI)1097-0061(19990915)15:12<1231::AID-YEA462>3.0.CO;2-V

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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