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Article

A functional 125-kDa core polypeptide of fission yeast DNA topoisomerase II.

Molecular and cellular biology - 1 Dec 1991

Shiozaki K, Yanagida M

Abstract excerpt

We purified fission yeast DNA topoisomerase II (topo II) to apparent homogeneity. It consists of a single 165-kDa polypeptide in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and, upon treatment with a bifunctional reagent, doubles its molecular weight. Limited proteolysis of intact topo II by papain produces a 125-kDa core, which lacks the N-terminal 75 and the C-terminal approximately 260 amino...

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Identifiers

PMID: 1658625
DOI: 10.1128/mcb.11.12.6093-6102.1991
PMCID: PMC361784

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