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Article

A functional 125-kDa core polypeptide of fission yeast DNA topoisomerase II.

Molecular and cellular biology - 1 Dec 1991

Shiozaki K, Yanagida M

Abstract excerpt

We purified fission yeast DNA topoisomerase II (topo II) to apparent homogeneity. It consists of a single 165-kDa polypeptide in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and, upon treatment with a bifunctional reagent, doubles its molecular weight. Limited proteolysis of intact topo II by papain produces a 125-kDa core, which lacks the N-terminal 75 and the C-terminal approximately 260 amino...

Topics

Amino Acid Sequence
Base Sequence
Cloning, Molecular
DNA Topoisomerases, Type II
DNA, Fungal
Electrophoresis, Polyacrylamide Gel
Endopeptidases
Genetic Complementation Test
Haploidy
Kinetics
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Papain

Schizosaccharomyces

Sequence Alignment

Source

Open in PubMed Open full text

Identifiers

PMID: 1658625
DOI: 10.1128/mcb.11.12.6093-6102.1991
PMCID: PMC361784

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1658625-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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