Article
Tripartite structure of Saccharomyces cerevisiae Dna2 helicase/endonuclease.
Nucleic acids research - 15 Jul 2001
Bae S H, Kim J A, Choi E, Lee K H, Kang H Y, Kim H D, Kim J H, Bae K H, Cho Y, Park C, Seo Y S
Abstract excerpt
In order to gain insights into the structural basis of the multifunctional Dna2 enzyme involved in Okazaki fragment processing, we performed biochemical, biophysical and genetic studies to dissect the domain structure of Dna2. Proteolytic digestion of Dna2 using subtilisin produced a 127 kDa polypeptide that lacked the 45 kDa N-terminal region of Dna2. Further digestion generated two subtilisin-resistant core...
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