Article
Function of the hydrophilic carboxyl terminus of type II DNA topoisomerase from Drosophila melanogaster. II. In vivo studies.
The Journal of biological chemistry - 5 Oct 1993
Crenshaw D G, Hsieh T
Abstract excerpt
Genetic complementation, protein distribution, and in vivo enzymatic activity by carboxyl-terminal truncation mutations of the Drosophila enzyme were examined. Removal of more than 273 of the 1447 amino acids composing the full-length topoisomerase inactivates the enzyme in vivo and in vitro; rem...
Topics
- Amino Acid Sequence
- Animals
- Chromosomes
- DNA Topoisomerases, Type II
- Drosophila melanogaster
- Fluorescent Antibody Technique
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Plasmids
- Saccharomyces cerevisiae
- Subcellular Fractions