Article
The topoisomerase I gene from Ustilago maydis: sequence, disruption and mutant phenotype.
Nucleic acids research - 11 Sept 1994
Gerhold D, Thiyagarajan M, Kmiec E B
Abstract excerpt
The Ustilago maydis genomic TOP1 gene encoding DNA topoisomerase I was cloned by amplifying a gene fragment using the polymerase chain reaction, and using this fragment to search a genomic DNA library by hybridization. The predicted peptide sequence exhibited 30-40% identity to other eukaryotic T...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- DNA Topoisomerases, Type I
- DNA, Fungal
- Fungal Proteins
- Gene Dosage
- Genes, Fungal
- Genomic Library
- Molecular Sequence Data
- Mutation
- Phenotype
- Promoter Regions, Genetic
- Sequence Alignment
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Ustilago