Article
Highly transformable mutants of Streptomyces aureofaciens containing restriction-modification systems.
Journal of basic microbiology - 1 Jan 1991
Muchová J, Lacová B, Godány A, Sevcíková B
Abstract excerpt
Streptomyces aureofaciens 13 is a mutant defective in chlortetracycline production. It was chosen as a potentially useful host for gene cloning in investigations of the organization of the biosynthetic genes for the tetracycline antibiotic pathway. From the Streptomyces aureofaciens 13 strain, three suitable clones were used for our work. The conditions for optimal formation and efficient transformation of...
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