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Article

Characterization of allelic replacement in Streptococcus parasanguis: transformation and homologous recombination in a 'nontransformable' streptococcus.

Gene - 16 Aug 1993

Fenno J C, Shaikh A, Fives-Taylor P

Abstract excerpt

We have obtained transformants of Streptococcus parasanguis FW213 containing allelic replacements in several chromosomal loci. Transformation occurred following electroporation with nonreplicating plasmids carrying two antibiotic-resistance-encoding genes, one of which is inserted into DNA homologous to the chromosomal target. In contrast with other streptococci, S. parasanguis FW213 is not transformed by linear...

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PMID: 8344531
DOI: 10.1016/0378-1119(93)90349-8

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