Article
Characterization of allelic replacement in Streptococcus parasanguis: transformation and homologous recombination in a 'nontransformable' streptococcus.
Gene - 16 Aug 1993
Fenno J C, Shaikh A, Fives-Taylor P
Abstract excerpt
We have obtained transformants of Streptococcus parasanguis FW213 containing allelic replacements in several chromosomal loci. Transformation occurred following electroporation with nonreplicating plasmids carrying two antibiotic-resistance-encoding genes, one of which is inserted into DNA homolo...
Topics
- Alleles
- Chromosomes, Bacterial
- DNA Mutational Analysis
- Genes, Bacterial
- Genetic Markers
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutagenesis, Site-Directed
- Plasmids
- R Factors
- Recombination, Genetic
- Restriction Mapping
- Sequence Homology, Nucleic Acid
- Streptococcus sanguis
- Transfection
- Transformation, Genetic