Article
Capture and release of DNA using aminosilane-modified bacterial magnetic particles for automated detection system of single nucleotide polymorphisms.
Biotechnology and bioengineering - 5 Aug 2006
Nakagawa Takahito, Hashimoto Reisuke, Maruyama Kohei, Tanaka Tsuyoshi, Takeyama Haruko, Matsunaga Tadashi
Abstract excerpt
Bacterial magnetic particles (BMPs) were modified with 3-[2-(2-aminoethylamino)-ethylamino]-propyltrimethoxysilane (AEEA) to produce a dense amine surface. Modification of BMPs in a toluene solution resulted in an increased amine yield, and approximately 11.3 x 10(4) surface amines were detected on a single particle. The modified BMPs were capable of efficient electrostatic capture of DNA. The maximum amount of...
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