Article
Development and evaluation of an automated workstation for single nucleotide polymorphism discrimination using bacterial magnetic particles.
Biosensors & bioelectronics - 15 Dec 2003
Tanaka Tsuyoshi, Maruyama Kohei, Yoda Kiyoushi, Nemoto Etsuo, Udagawa Yuuji, Nakayama Hideki, Takeyama Haruko, Matsunaga Tadashi
Abstract excerpt
We designed an automated workstation for magnetic particle-based single nucleotide polymorphism (SNP) discrimination of ALDH genotypes. Bacterial magnetic particles (BMPs) extracted from Magnetospirillum magneticum AMB-1 were used as DNA carriers. The principle for SNP discrimination in this study was based on fluorescence resonance energy transfer (FRET) between FITC (donor) and POPO-3 (acceptor) bound to...
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