An atypical approach identifies TYR234 as the key base catalyst in chondroitin AC lyase.

Abstract excerpt

Chondroitin AC lyase from Flavobacterium heparinum catalyses the degradation of chondroitin by an anionic E1cb elimination mechanism that involves proton abstraction from C5 of glucuronic acid. The lyase also carries out efficient proton transfer to a sugar nitronate anion, which was designed originally as an inhibitor of the enzyme, with a second-order rate constant of kcat/Km=2.7x10(6) M(-1) s(-); this is very...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Binding Sites
• Share to Carbohydrate Conformation