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Article

Role of His-His interaction in Ser474-His475-Tyr476 sequence of chondroitinase ABC I in the enzyme activity and stability.

International journal of biological macromolecules - 1 Apr 2018

Maleki Monireh, Khajeh Khosro, Amanlou Massoud, Golestani Abolfazl

Abstract excerpt

Despite clinical importance of chondroitinase ABC I, its application has been limited due to thermal instability as reported in the literature. There are various approaches to improve thermal stability of enzymes, among them, His-His interactions are believed generally as an effective means. In the present study and for preparing a His-His interaction, various mutations in the sequence of Ser474-His475-Tyr476 at...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 29146558
DOI: 10.1016/j.ijbiomac.2017.11.075

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