Semi-rational design of the N-terminal of Chondroitinase ABC to improve the catalytic activity.

Abstract excerpt

Chondroitinase ABC (ChSase ABC), which can digest chondroitin sulfate (CS), has been utilized for the analysis of CS glycan structure and the preparation of low-molecular-weight chondroitin sulfate. However, the catalytic activity of most ChSase ABCs remains insufficient for industrial or large-scale applications. Previous structure studies of ChSase ABCs indicate that the N-terminal domain is typically involved...

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• Bacteroides
• Chondroitin Sulfates
• Hydrolysis
• Kinetics
• Mutagenesis, Site-Directed