Article
Catalytic mechanism of heparinase II investigated by site-directed mutagenesis and the crystal structure with its substrate.
The Journal of biological chemistry - 25 Jun 2010
Shaya David, Zhao Wenjing, Garron Marie-Line, Xiao Zhongping, Cui Qizhi, Zhang Zhenqing, Sulea Traian, Linhardt Robert J, Cygler Miroslaw
Abstract excerpt
Heparinase II (HepII) is an 85-kDa dimeric enzyme that depolymerizes both heparin and heparan sulfate glycosaminoglycans through a beta-elimination mechanism. Recently, we determined the crystal structure of HepII from Pedobacter heparinus (previously known as Flavobacterium heparinum) in complex with a heparin disaccharide product, and identified the location of its active site. Here we present the structure of...
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