Article
Determination of thiopurine S-methyltransferase (TPMT) activity by comparing various normalization factors: reference values for Estonian population using HPLC-UV assay.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences - 13 Apr 2006
Oselin Kersti, Anier Kaili, Tamm Riin, Kallassalu Kristi, Mäeorg Uno
Abstract excerpt
Thiopurine S-methyltransferase (TPMT; EC 2.1.1.67) is the key enzyme in the metabolism of thiopurine drugs. Determination of TPMT activity has been used for the individualization of thiopurine dose. We developed HPLC-UV assay for the determination of TPMT activity in human erythrocytes using 6-mercaptopurine as a substrate. Various extraction and chromatographic conditions were compared. In-house developed...
Topics
- Chromatography, High Pressure Liquid
- Estonia
- Genetics, Population
- Genotype
- Humans
- Methyltransferases
- Reference Standards
- Spectrophotometry, Ultraviolet